논문업데이트

Home. 최근뉴스. 논문업데이트

논문업데이트

Atopic donor status does not influence the uptake of the major grass p…

페이지 정보

작성자 관리자
댓글 0건 조회 1,330회 작성일 15-07-04 11:52

본문

J Immunol Methods. 2015 Jun 6. pii: S0022-1759(15)30003-X. doi: 10.1016/j.jim.2015.06.001. [Epub ahead of print]

Atopic donor status does not influence the uptake of the major grass pollen allergen, Phl p 5, by dendritic cells.

Ashjaei K1, Palmberger D2, Bublin M3, Bajna E4, Breiteneder H5, Grabherr R6, Ellinger I7, Hoffmann-Sommergruber K8.
 
Abstract
Dendritic cells (DCs) are sentinels of the immune system for antigen recognition and uptake, as well as presentation to naïve T cells for stimulation or priming. Internalization and endocytic degradation of allergens by DCs are important steps required for T cell priming. In the current study we investigated binding and internalization of purified recombinant non-glycosylated grass pollen allergen, Phl p 5, and natural non-specific lipid transfer protein from sunflower, SF-nsLTP to human monocyte derived dendritic cells (MoDCs). Colocalization of Phl p 5 with low affinity (CD23) or high affinity receptor (FcεRI) was investigated by immunofluorescence staining. Likewise, localization of the allergens in early (EE) and late endosomes (LE) was detected by co-staining for early endosome antigen (EEA1) and lysosomal-associated membrane protein 1 (LAMP1). In our experimental setting we could demonstrate that Phl p 5 as well as SF-nsLTP bound to MoDCs from both, grass pollen allergic and non-allergic individuals. Competitive allergen uptake experiments demonstrated non-preferential and simultaneous uptake of Phl p 5 and SF-nsLTP by MoDCs. No overlap of signals from Phl p 5 and CD23 or FcεRI was detectable, excluding IgE-mediated uptake for this allergen. Both allergens, Phl p 5 and SF-nsLTP, were localized in early and late endosomes. The present study applied a set of methods to assess the allergen uptake by MoDCs in an in vitro model. No qualitative and quantitative differences in the allergen uptake of both, Phl p 5 and SF-nsLTP were detected in single and competitive assays.
  • 목록

    • 댓글목록

    등록된 댓글이 없습니다.