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Persistence of the IgE repertoire in birch pollen allergy

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Persistence of the IgE repertoire in birch pollen allergy
Otte, Manuel; Mahler, Vera; Kerpes, Andrea; Pabst, Oliver
A similar approach was recently used to reveal overlapping IgE repertoires between nasal biopsies and blood samples of patients with allergic rhinitis.8 The 3 female patients were of similar age (50-59 years old) and characterized by high serum IgE CAP fluorescent enzyme immunoassay (FEIA) (class 4-6) to birch pollen extract or CAP FEIA class 5 or higher for the major birch pollen allergen Bet v 1. To determine the complexity of the IgE repertoire in comparison to other immunoglobulin isotypes in these patients, we isolated RNA from PBMCs and generated RT-PCR products with 5' primers specific for the leader region in immunoglobulin VH1, VH3, and VH4 genes, which cover the vast majority of the expressed human VH genes, and a set of 3' primers binding in the first exon of the constant region of different CH genes as described in this article's Methods section in the Online Repository at www.jacionline.org.
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