논문업데이트

Home. 최근뉴스. 논문업데이트

논문업데이트

Involvement of cross-reactive carbohydrate determinants-specific IgE i…

페이지 정보

작성자 관리자
댓글 0건 조회 1,358회 작성일 17-08-31 20:33

본문

Involvement of cross-reactive carbohydrate determinants-specific IgE in pollen allergy testing
Hidenori Yokoi, Hiroshi Yoshitake, Yuma Matsumoto, Michitsugu Kawada, Yoshiki Takato, Kiyomi Shinagawa, Hiroyuki Sakurai, and Koichiro Saito
Background<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />

Specific IgE antibodies against the low-molecular-weight carbohydrate antigen that does not bridge IgE molecules on mast cells are not associated with clinical symptoms. Cross reactivity can be determined in allergen-specific IgE detection assays when the carbohydrate structures between pollen allergens and plant derived food allergens are similar; in such cases, false positive results for grain or legume allergens can be reported for pollen allergic patients who are not sensitized to those allergens. This phenomenon arises owing to the presence of cross-reactive carbohydrate determinants (CCDs).

Objective

This study aimed to assess the impact of CCD interference on the results for pollen allergen-specific IgE antibodies in the general adult population and to perform CCD inhibition tests evaluating the involvement of CCD on samples positive to pollen allergens.

Methods

Serum samples from 322 subjects were tested for IgE antibodies to pollens and CCD. The research subjects were given questionnaires about pollen allergic symptoms to help assess the presence of allergies. Allergen IgE antibodies for Japanese cedar, Japanese cypress, orchard grass, ragweed, MUXF, bromelain, horseradish peroxidase (HRP), and ascorbate oxidase (ASOD) were analyzed.

Results

It was observed that among individuals who tested positive to any of the pollen allergens, the positive ratio of CCD-specific IgE antibody was the highest for HRP (13.5%–50.0%). The results from the inhibition tests revealed that CCD was marginally present. Although IgE antibodies for cedar pollen did not react with CCD, IgE antibodies for Japanese cypress, orchard grass, and ragweed might be detected by the presence of CCD.

Conclusion

The results of the inhibition tests revealed the obvious presence of CCD suggesting its involvement. Considering these findings, careful evaluation of patient IgE results should be performed for Japanese cypress, orchard grass, and ragweed.

 
  • 목록

    • 댓글목록

    등록된 댓글이 없습니다.