논문업데이트

Home. 최근뉴스. 논문업데이트

논문업데이트

Prevention of allergic conjunctivitis in mice by a rice-based edible v…

페이지 정보

작성자 관리자
댓글 0건 조회 1,477회 작성일 15-02-04 15:57

본문

Br J Ophthalmol. 2015 Jan 6. pii: bjophthalmol-2014-305842. doi: 10.1136/bjophthalmol-2014-305842. [Epub ahead of print]

Prevention of allergic conjunctivitis in mice by a rice-based edible vaccine containing modified Japanese cedar pollen allergens.

Fukuda K1, Ishida W1, Harada Y1, Wakasa Y2, Takagi H2, Takaiwa F2, Fukushima A1.

Author information

  • 1Department of Ophthalmology and Visual Science, Kochi Medical School, Nankoku City, Kochi, Japan.
  • 2Functional Transgenic Crop Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.

Abstract

BACKGROUND/AIMS:

To determine whether oral immunotherapy with transgenic rice seeds expressing hypoallergenic modified antigens suppresses cedar pollen-induced allergic conjunctivitis by eliciting immune tolerance in mice.

METHODS:

BALB/c mice were fed once a day for 20 days with 220 mg of transgenic rice expressing modified Japanese cedar pollen allergens Cry j 1 and Cry j 2 or with non-transgenic rice seeds as a control. They were then sensitised with two intraperitoneal injections of Japanese cedar pollen in alum before challenge twice with pollen in eye drops. Twenty-four hours after the second challenge, the conjunctiva, spleen, and blood were isolated for histological analysis, cytokine production assays, and measurement of serum immunoglobulin E concentrations, respectively.

RESULTS:

The numbers of eosinophils and total inflammatory cells in the conjunctiva were significantly lower in mice fed the transgenic rice than in those fed non-transgenic rice. The clinical score evaluated at 15 min after antigen challenge was also significantly lower in mice fed the transgenic rice than in those fed non-transgenic rice. The serum concentrations of both total and allergen-specific immunoglobulin E were also significantly lower in mice fed the transgenic rice. Oral vaccination with transgenic rice resulted in significant down-regulation of the allergen-induced production of interleukin (IL)-2, IL-4, IL-5, IL-12p70, interferon-γ, and IL-17A by splenocytes.

CONCLUSIONS:

Oral immunotherapy with transgenic rice expressing modified Japanese cedar pollen allergens suppressed pollen-induced experimental allergic conjunctivitis in mice by eliciting immune tolerance. This novel prophylactic approach is potentially safe and effective for allergen-specific oral immunotherapy in allergic conjunctivitis.
Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

KEYWORDS:

Conjunctiva; Immunology; Ocular surface; Treatment other
  • 목록

    • 댓글목록

    등록된 댓글이 없습니다.